Oroxylum indicum Seeds—Analysis of Flavonoids by Micellar Electrokinetic Chromatography
نویسنده
چکیده
Flavonoids are bioactive constituents in Oroxylum indicum seeds, an Asian traditional remedy used for the treatment of respiratory infections. In this study the first capillary electrophoretic method for their determination is presented. By using a 25 mM borax buffer at pH 9.2 containing 10 mM SDS as detergent, the determination of seven flavonoids was feasible in only 13 min. Method validation confirmed that the assay is in accordance with ICH requirements in respect to linearity, selectivity, sensitivity, accuracy and precision. Quantitative results revealed that baicalein-7-O-gentiobioside is the most abundant flavonoid in the drug (1.19 to 5.33%), followed by other baicalein derivatives (7-O-glucoside, 7-O-glucuronide). These observations were in good qualitative and quantitative agreement with LC-MS results.
منابع مشابه
Screening for Antibacterial and Antioxidant Activities and Phytochemical Analysis of Oroxylum indicum Fruit Extracts.
Oroxylum indicum, which is called Pheka in Thai, is a traditional Thai plant in the Bignoniaceae family with various ethnomedical uses such as as an astringent, an anti-inflammatory agent, an anti-bronchitic agent, an anti-helminthic agent and an anti-microbial agent. The young fruits of this plant have also been consumed as vegetables. However, there has been no report concerning its antibacte...
متن کاملPhytochemical Screening and Evaluation of Analgesic Activity of Oroxylum indicum
We aimed to study phytochemical screening and analgesic activity of ethanol extract of Oroxylum indicum. The dried powder of the barks of the plant was extracted with 95% ethanol and was subjected to various phytochemical tests to ascertain the principle constituents contained in the extract. The result revealed the presence of alkaloids, flavonoids, tannins, glycosides in the ethanol extract o...
متن کاملPhytopharmacogostical Studies on Root Bark of Oroxylum Indicum, Vent
The root bark of Oroxylum indicum, vent (Syonakh) belonging to family Bignoniaceae, are reported to have a great medicinal value. Pharmacognostical evaluation including examination of morphological and microscopical characters, determination of quality control parameters such as ash values, extractive values, moisture content, foreign matter, and microbial contamination were carried out. Phytoc...
متن کاملChemical Constituents from Oroxylum Indicum (l.) Kurz of Nepalese Origin
Oroxylum indicum commonly called Indian tumplet flower (Nepali name: Tatelo, Karamkanda, saune tatal) belongs to the Bignoniaceae family and it is widely used in traditional medicine.1, 2 Traditionally, the stem, bark, seeds and leaves used for the treatment of the diarrhea, typhoid and stomachache. It has also been used as antitussive, analgesic and anti-inflammatory agents for the treatment o...
متن کاملEvaluation of variation of acteoside and three major flavonoids in wild and cultivated Scutellaria baicalensis roots by micellar electrokinetic chromatography.
Micellar electrokinetic chromatography (MEKC) conditions were developed to analyze the constituents of Scutellariae Radix (SR) and Scutellaria baicalensis roots. Using the MEKC method, the major flavonoid constituents of baicalin, baicalein and wogonin of wild and cultivated S. baicalensis roots were compared. In a preliminary comparison of electropherogram, one special peak was found in a wild...
متن کامل